New E-Plate Insert for Analysis of Cell-Cell Interactions using the Roche xCELLigence System
13 Jun 2012

Roche Applied Science is pleased to announce the launch of the new E-Plate insert for cell-cell interactions. This is based on research suggesting that the co-cultivation of two different cell types is an important way to investigate cell-cell interactions and communication.

Co-cultivation can be used in a wide variety of applications, including cell signalling and toxicology testing. A key goal in drug discovery is to identify how to most effectively monitor cell-cell interactions when cells show pathology. Roche Applied Science is now providing the new E-Plate Insert 16 and E-Plate Insert 96, permitting continual impedance-based monitoring of indirect cell-cell interactions/communication using xCELLigence Instruments.

In a recent study at the INRS-Institut Armand-Frappier (Laval, Quebec, Canada) the E-Plate Insert had been used to test the behaviour of BeWo human placental choriocarcinoma cells in co-culture with H295R human adrenocortical carcinoma cells. “Using the E-Plate Insert in our co-culture experiments requires fewer manipulations and allows us to perform several experiments simultaneously - hormone measurements, signalling pathway analysis, real-time monitoring of biochemical and morphological differentiation of the cells,” summarized Prof. Thomas Sanderson and Prof. Cathy Vaillancourt, who are co-investigators on this project and co-supervisors of Andrée-Anne Hudon Thibeault, the Masters student who realised the study.

The Canadian group plans to use the unique abilities of the co-culture-compatible E-Plate Insert and xCELLigence cell response monitoring technology, to determine the effect of co-culture on primary villous trophoblast homeostasis (polarization, differentiation/syncytialization, fusion, apoptosis) and on H295R cells (proliferation, viability, apoptosis) as well as expression and activity levels of key placental and fetal adrenal steroidogenic enzymes.

The membrane-containing E-Plate Inserts enable sensitive, reproducible measurement of indirect cell-to-cell interactions in real-time, and in a label-free environment using the xCELLigence Instruments (RTCA DP, SP, and MP Analyzers). Combined with microscopy-compatible E-Plates VIEW, E-Plate Inserts 16 and 96 are available for low to high throughput applications. Impedance-based measurements are particularly advantageous when co-culturing cells for pinpointing when and how cells respond to each other, thus identifying the best time points for further experiments.

For more information please view the company website.

Request Info


Company website

Roche Applied Science - a member of the Roche Group