JM Science to Showcase Shiseido HPLC Columns for Protein Separation at Pittcon 2014
11 Dec 2013

Chromatographic separations of proteins are based on their difference either in size (size exclusion chromatography), electric charges (ion exchange chromatography), or hydrophobicity (reversed-phase chromatography). JM Science offers a complete line of Shiseido HPLC CAPCELL PAK Columns with the developed Proteonavi to separate proteins and peptides in reversed phase mode. In reversed-phase mode, it is known that proteins and peptides with higher-order structure are denatured to some extent in the course of being retained on the stationary phase after introduction to the column. Once the retention with denaturation is occurred, molecules will not migrate until the organic content of the mobile phase is raised to an appropriate level under a gradient program.

Proteonavi utilizes high-purity silica with few metal impurities, and shows minimal irreversible adsorption for proteins and peptides. Its pore size is as wide as 30 nm, so that large proteins are able to have enough interactions with the stationary phase.

The primary source of protein retention on reversed phase is hydrophobic interaction between hydrophobic parts of amino acids residues and alkyl chains in the stationary phase. When secondary interactions, such as coulombic interactions by acidic silanols or metal impurities in the stationary phase, exist to a large extent, peak shapes of proteins will be deteriorated due to their slow kinetics. Proteonavi shows better peak profiles and higher resolution among standard proteins, in comparison with conventional wide-pore columns.

Proteonavi is suitable to large-scale preparative separation, taking advantage of its low-column bleed nature and high separation efficiency.

JM Science will be exhibiting these new HPLC CAPCELL PAK Columns at PITTCON 2014 at Booth# 2802.

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Sarah Thomas
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