Bio-Rad Introduces Mini-PROTEAN TGX Stain-Free Precast Gels in Gradient Format
13 Sep 2012

Bio-Rad Laboratories, Inc. has announced the launch of its Mini-PROTEAN TGX (Tris-glycine extended) Stain-Free precast gels in gradient formats 4–15% and 4–20%, which are ideal for samples that contain a broad range of protein sizes. The new formats extend Bio-Rad’s line of mini- and midi-sized stain-free precast gels, providing researchers with the fastest separation and in-gel protein visualization capabilities available for Laemmli-based PAGE.

Typical polyacrylamide gel staining methods that follow Coomassie protocols can take four or more hours to complete. The Mini-PROTEAN TGX Stain-Free precast gels include a unique trihalo compound that allows rapid fluorescent detection of proteins in gels or on membranes without the need for staining. When researchers use the Mini-PROTEAN TGX Stain-Free precast gels as part of Bio-Rad’s V3 Western Workflow™, they can achieve complete protein separation, gel imaging, and data analysis in just 20 minutes.

Other key benefits of Mini-PROTEAN TGX Stain-Free precast gels in gradient format include:

• Greater transfer efficiency — proteins can be transferred onto PVDF or nitrocellulose membranes in as little as 3 minutes with the Trans-Blot® Turbo™ system.

• Versatility — the same gel can be used for western blotting, standard staining methods, and mass spectrometry analysis of proteins.

• Cost-effectiveness — unlike other precast gels that require specialized buffer systems and stains, the Mini-PROTEAN TGX Stain-Free precast gels use inexpensive standard Laemmli buffer and do not require staining and destaining, keeping running costs low.

The Mini-PROTEAN TGX Stain-Free precast gels are available in a wide range of percentages: 7.5%, 10%, 12%, 4–15%, 4–20%, and Any kD™ (offers optimal resolution in the 10–100 kD range). Gels can be stored for up to 12 months without loss of performance.

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