Bio-Rad’s New Immun-Blot® LF PVDF Membranes Improve Fluorescent Blot Detection
04 Oct 2011

Bio-Rad Laboratories, Inc. announces the launch of the Immun-Blot LF (low fluorescence) PVDF membranes; Protein blotting membranes optimized for fluorescence and multiplex fluorescence applications. The membranes offer high signal-to-background ratio, low autofluorescence, and superior protein retention to maximize blot detection sensitivity and enable downstream quantitation.

Two primary types of protein blotting membranes are used in western blotting applications: nitrocellulose and PVDF (polyvinylidene fluoride). While both types are suitable for colorimetric and chemiluminescent detection, their high autofluorescence and lower signal-to-background ratios are not suitable for fluorescent detection.

Immun-Blot LF PVDF membranes are a low-fluorescence alternative specifically designed for fluorescent immunoblotting and detection in the visible light (red/green/blue) spectrum. Immun-Blot LF PVDF membranes produce highly sensitive and quantitative results thanks to their superior signal-to-background ratios, which are three times higher than those of nitrocellulose and conventional PVDF.

Key Features
• Low autofluorescence and high signal-to-background ratio – produce a stronger signal, more accurate protein quantitation, and higher quality images
• Superior protein retention – offers binding capacity of 300 µg/cm2 and improves protein retention, especially of low-abundance proteins
• Precut membrane and filter paper sets – save time and eliminate excessive membrane handling and potential contamination

Immun-Blot LF PVDF membranes are compatible with any imager possessing visible RGB excitation channels, including Bio-Rad’s new ChemiDoc™ MP imager, which allows researchers to visualize proteins at every stage of a blotting experiment when paired with Bio-Rad’s proprietary Stain-Free technology. The membranes are also compatible with a wide range of fluorescent labels, such as DyLights, Q-Dots, and Alexa fluorophores, as well as colorimetric and chemiluminescent detection methods.

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