AnaSpec Highlights HiLyte Fluor™ dyes Collection
02 Mar 2012AnaSpec is pleased to highlight a wide collection of amine and thiol reactive 6-isomeric dyes of fluoresceins and rhodamines, and AnaSpec’s own proprietary HiLyte Fluor™ dyes for labeling amine and thiol modified oligonucleotides.
Fluorescent dye-labeled oligos are widely used as primers for sequencing and PCR reactions, and also as probes for fluorescence in situ hybridization (FISH). Double-labeled oligos are used to produce fluorescence resonance energy transfer (FRET) for a number of applications such as real-time PCR assays and automated DNA sequencing.
Synthetic oligos can be modified with amine or thiol groups at their 5’-end, 3’-end or internally. These amine and thiol incorporated oligos can then be labeled with amine and thiol reactive fluorophores, respectively.
N-hydroxysuccinimidyl esters (SE) and isothiocyanates of fluorescein and rhodamine derivatives are used for labeling amine-modified oligos. Maleimides and iodoacetamides of fluorescein and rhodamine derivatives are used for labeling thiol-modified oligos.
AnaSpec’s proprietary HiLyte Fluor™ dyes are available in the SE and maleimide forms. These dyes have fluorescence emission wavelengths spanning the full visible and near infrared (NIR) spectrum. HiLyte Fluor™ dyes have properties that are superior to conventional dyes, with high fluorescence quantum yields, large extinction coefficients, good photostability, low pH sensitivity and improved water solubility.
AnaSpec’s HiLyte Fluor™ dyes and classic dyes are excellent choices for labeling amine- and thiol-modified oligonucleotides.
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