Automation of a Homogeneous Proximity Assay for Detection of Residual Protein A in Biological Therapeutics
3 Dec 2014

Protein A is used in affinity chromatography for therapeutic antibody purification and has been shown to leach from columns during purification of antibodies resulting in contamination. Residual Protein A in therapeutic drugs could elicit mitogenic or immunological reactions in patients. This application note demonstrates automation of a HTS compatible homogenous proximity assay for the presence of residual Protein A in biological therapeutics using AlphaLISA technology.

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