Expression Levels of Drug-metabolizing Enzyme, Transporter and Nuclear Receptor mRNAs in a Novel Three-dimensional Culture System for Human Hepatocytes using Micro-space Plates
30 March 2017
In this study, the mRNA expression levels of many target genes are measured in four lots of cryopreserved human hepatocyte cells to compare mRNA expression levels in cultures using traditional plates with micro-space plates, such as Elplasia. The results of the study show that culturing hepatocytes in micro-space plates induces a significantly smaller change in mRNA expression relative to the value before inoculation of the hepatocytes. In vitro three-dimensional culture systems may be useful for evaluating pharmacokinetics, pharmacodynamics, and toxicity of drug candidates and biogenic substances.