Absolute Real-Time PCR: A Comparison of Spectrophotometric and On-Chip Methods for External Standard Curve Construction from Different Nucleic Acid Dosages
27 Jan 2015

Concentration determination of DNA using real-time quantitative PCR technology plays a substantial role for identification and quantitation of microbes in unknown samples in many research areas. This Application Note focuses on establishing calibration standards by two different approaches – spectrophotometry and on-chip electrophoresis. The Agilent 2100 Bioanalyzer system provides accurate and precise quantitation of DNA, enabling the preparation of reliable standard curves for absolute quantitation with qPCR assays.

Show    per page
4 of 14     Page  ... 4 5 6  ... Next 
High-Throughput Cloning of Functional Elements in the Human Genome DNA, high throughput, DNA amplification22 Sep 2014Read
Direct PCR from Plant Material is a Challenging Application as a Result of the Diversity of Plant Tissue Types and the Potent PCR Inhibitors Contained within the Tissue PCR purification, PCR automation20 Sep 2014Read
Fully Automated Purification of Viral Nucleic Acids and Detection with Ready-to-Use artus® PCR Kits nucleic acid concentration, pathogen detection20 Sep 2014Read
Fast qPCR with Takara SYBR® Premix Ex Taq qPCR, real-time pcr19 Sep 2014Read
Amplification of Long Products from Human Genomic DNA DNA, enzymes, Genome Sequencing19 Sep 2014Read
KAPA2G™ Fast HotStart Multiplex PCR PCR purification, PCR automation19 Sep 2014Read
KAPA2GTM Robust HotStart Mouse Genotyping qPCR, DNA fragment sizing, Genome Sequencing12 Sep 2014Read
Development of the PowerPlex® Fusion System multiplex, DNA, DNA amplification10 Sep 2014Read
Considerations for Use of Taq Polymerase in Molecular Diagnostic Assays qPCR, assay development, enzymes10 Sep 2014Read
Straightforward PCR Optimization and Highly Flexible Operation on the Dual Block Thermocycler Mastercycler Nexus GX2 qPCR27 Aug 2014Read

Popular tags in SelectScience