Engineered DNA Polymerases Improve NGS and Enable Novel Applications
24 Jul 2014

The vast majority of PCR applications utilize a small number of wild-type DNA polymerases; improvements to existing applications and the development of novel PCR applications have primarily been realized via innovative changes to all of the reaction components except the polymerases themselves. More recently, KAPA have engineered a new generation of improved DNA polymerases for PCR applications, including KAPA HiFi, a high-fidelity, proof-reading DNA polymerase. KAPA HiFi is capable of robust amplification of diverse targets spanning a wide range of sizes and GC-content, and KAPA have demonstrated that KAPA HiFi produces high yields with remarkably little amplification bias when used for NGS library amplification. With improved Methyl-seq performance in mind, KAPA further engineered KAPA HiFi to allow the efficient amplification of bisulfite-converted DNA libraries containing uracil. Using a number of case studies, it is illustrated that these engineered DNA polymerases are providing better sequence coverage and are enabling novel and challenging NGS applications.

Show    per page
1 of 4     Page  1 2 3  ... Next 
Discrimination of Sturgeon and Related Species by PCR-RFLP Using the Agilent 2100 Bioanalyzer System food safety, food contamination, seafood9 Jun 2014Read
High-throughput Miniaturized Quantitative PCR with the Echo® 525 Liquid Handler qPCR, liquid handling workstation, PCR automation28 May 2014Read
Direct PCR from Blood Preserved on Whatman FTA and 903 Cards using Thermo Scientific Phusion Blood Direct PCR Kit qPCR, DNA amplification, FTA card punching6 Feb 2014Read
Enhancing Forensic Analysis of Trace DNA Using the MinElute® PCR Purification Kit DNA amplification, forensic investigation5 Dec 2013Read
Probing Copy Number Variations Using Bio-Rad’s QX100™ digital pcr, copy number variation5 Dec 2013Read
Comparative Run Time Evaluations of PCR Thermal Cyclers qPCR, Mastercycler Nexus, RT qPCR3 Dec 2013Read
Monitoring Differential Expression in Stem Cells qPCR, stem cell differentiation, RT qPCR22 Nov 2013Read
QX100™ Droplet Digital™ PCR System for Accurate Quantification of Illumina TruSeq Libraries ddPCR, NGS22 Nov 2013Read
Reliable mRNA Expression Profiles with FFPE Tissues Using the Agilent SurePrint G3 Human Gene Expression v2 8x60K Microarray qPCR, FFPE extraction, FFPE21 Nov 2013Read
SsoFast™ EvaGreen® Supermix Stability for Automated qPCR qPCR, PCR automation, real-time pcr21 Nov 2013Read

Popular tags in SelectScience