Absolute Real-Time PCR: A Comparison of Spectrophotometric and On-Chip Methods for External Standard Curve Construction from Different Nucleic Acid Dosages
27 January 2015
Concentration determination of DNA using real-time quantitative PCR technology plays a substantial role for identification and quantitation of microbes in unknown samples in many research areas. This Application Note focuses on establishing calibration standards by two different approaches – spectrophotometry and on-chip electrophoresis. The Agilent 2100 Bioanalyzer system provides accurate and precise quantitation of DNA, enabling the preparation of reliable standard curves for absolute quantitation with qPCR assays.