Metabolic Incorporation of Stable Isotope Labels into Glycans
2 August 2017

In this note, an in vivo labeling strategy for glycomic studies is described, analogous to the SILAC approach used for proteomics. This glycomic methodology is called IDAWG – isotopic detection of aminosugars with glutamine – and is based on the side-chain of glutamine being the sole nitrogen donor source in the biosynthesis of aminosugar nucleotides.