Fast and High-Resolution Reversed-Phase Separation of Synthetic Oligonucleotides
20 August 2015

This application note demonstrates fast and high-resolution separation and identification of a number of de-protected (removal of the dimethoxytrityl group) DNA and RNA oligonucleotides, using Agilent AdvanceBio Oligonucleotide columns. Synthetic DNA- and RNA-based oligonucleotides are among many successful biotherapeutic drugs to treat many different kinds of illnesses. To ensure drug potency, and to reduce the potential for drug interactions, a high-purity product is required, therefore, analyzing the purity of the products is important.