Fast and Convenient 5-Hydroxymethylcytosine Enrichment Workflow for Next-Generation Sequencing
22 July 2015
5-hydroxymethylcytosine (5-hmC) is an extensively studied DNA epigenetic modification, of which number of its important epigenetic functions such as gene regulation and X chromosome imprinting are known. Improved enrichment methods took analysis of epigenetic modification to a new level, and a number of enrichment kits are now available. This application notes illustrates the high specificity of the Scientific™ EpiJET™ 5-hmC Enrichment Kit, and demonstrates how it can be used for both locus-specific and whole genome–specific analysis, yielding 5-hmC distribution patterns across different genomes.