Microvolume Quantification of Nucleic Acids in Molecular Diagnostics with Thermo Scientific Nanodrop
28 Oct 2014
Quantification of nucleic acid samples prior to analysis is an important QC step in order to verify suitability for downstream testing. The Thermo Scientific NanoDrop 2000c and NanoDrop 8000 present several key advantages over conventional spectrophotometers, particularly the capability to quantify nucleic acids in as little as a single microliter. Analysis takes less than five seconds per sample, while the exceptional dynamic range of the instruments means that sample dilution is not necessary. Here we describe the necessity of microvolume quantification of nucleic acids prior to downstream molecular diagnostic applications such as qPCR, RT-qPCR, STR analysis, SNP genotyping and DNA sequencing, and also describe how the NanoDrop™ technology provides unique advantages over other quantification methods.