Automated Monitoring of Metabolic Activity and Differentiation of Human Mesenchymal Stem Cells
26 October 2016
Human mesenchymal stem cells (MSCs) are multipotent adult stem cells with a high capacity for self-renewal originating from perivascular progenitor cells. Using appropriate differentiation media, MSCs can be differentiated in vitro towards adipocytes. This application note describes the results of a study aimed at identifying phenotypic changes during MSC differentiation by automated, real-time analysis using the Spark 10M multimode reader and several well-established and reliable cell-based assays for the assessment and quantification of cell viability, cytotoxicity and apoptosis developed by PromoCell.