The Analysis of Vitamin D Metabolites in Serum/Plasma by LC‐MS/MS
10 October 2017

Vitamin D exists in two forms, vitamin D2 and vitamin D3. Each undergoes metabolism to form 25‐hydroxyvitamin D2 and 25‐hydroxyvitamin D3. For accurate determination of vitamin D levels in the blood, it is important to distinguish between these metabolites and to separate them from major matrix interferences. In this method, the RaptorTM ARC‐18 column combines the speed of superficially porous particles (SPP) with the resolution of highly selective USLC® technology to produce a simple and rugged method for the determination of vitamin D metabolites in serum and plasma.