Efficient Uniform Labeling of Proteins Expressed in Baculovirus-Infected Insect Cells Using BioExpress® 2000 Insect Cell Medium
23 July 2017

Uniform isotope labeling is a key tool for NMR studies on recombinant proteins and their interaction with ligands of pharmaceutical interest. To be useful for most important NMR studies, a labeled protein has to be expressed with baculovirus-infected insect cells in the labeling medium in sufficient quality and quantity for isolation of at least 10 mg of correctly folded protein. This application note describes a method for efficiently labeling these proteins.