Separation of Polyphenols by Comprehensive 2D-LC and Molecular Formula Determination by Coupling to Accurate Mass Measurement
19 Aug 2014
This application note demonstrates the coupling of an Agilent 1290 Infinity 2D-LC Solution to an Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight LC/MS instrument for the measurement of polyphenolic compounds. The compounds separated by comprehensive 2D-LC are detected by the mass spectrometer and their accurate mass is measured. The compounds are identified by the LC Image LC × LC-HRMS software package. It is shown that their mass can be measured with high accuracy for the determination of their molecular formula.