High Content RNAi Screening using the Cytation™ 5 Cell Imaging Multi-Mode Reader to Image Printed RNAi Libraries from Persomics
12 February 2016
RNAi knockdown experiments offer the ability to test the physiologic role of individual gene products through loss of function. Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled genome-wide functional screens in cultured cells. These screens have resulted in the identification of new cellular pathways and potential drug targets. Due to the enormous number of genes, hundreds of plates and copious amounts of reagents are required to perform a genome wide screen with redundancy to provide adequate statistics. This application note demonstrates the use of a technology that miniaturizes the assay such that the same screen can be performed with only a handful of specialized plates.