Xpose Reader App: Quantification of Purified PCR Samples with Spectral Content Profiling
27 March 2015

An app for specific quantification of DNA amplicon in purified PCR samples is available on the Xpose ‘Touch & Go’ reader. Trinean’s proprietary spectral content profiling is used to isolate the profile of the desired molecule (here DNA) from the measured UV/VIS spectrum, thereby distinguishing it from absorbing sample contaminants. The specific DNA profile is then used to determine the DNA concentration. This application note describes how to use this application and how to interpret its results.