Engineered DNA Polymerases Improve NGS and Enable Novel Applications
24 Jul 2014

The vast majority of PCR applications utilize a small number of wild-type DNA polymerases; improvements to existing applications and the development of novel PCR applications have primarily been realized via innovative changes to all of the reaction components except the polymerases themselves. More recently, KAPA have engineered a new generation of improved DNA polymerases for PCR applications, including KAPA HiFi, a high-fidelity, proof-reading DNA polymerase. KAPA HiFi is capable of robust amplification of diverse targets spanning a wide range of sizes and GC-content, and KAPA have demonstrated that KAPA HiFi produces high yields with remarkably little amplification bias when used for NGS library amplification. With improved Methyl-seq performance in mind, KAPA further engineered KAPA HiFi to allow the efficient amplification of bisulfite-converted DNA libraries containing uracil. Using a number of case studies, it is illustrated that these engineered DNA polymerases are providing better sequence coverage and are enabling novel and challenging NGS applications.

Show    per page
1 of 96     Page  1 2 3  ... Next 
Consumable Effects on Low Volume TaqMan® Assays for Quantitative PCR on Roche LightCycler® 480 qPCR24 Jul 2014Read
Fast and Efficient HILIC Methods for Improved Analysis of Complex Glycan Structures biopharmaceuticals, chromatography, glycosylation24 Jul 2014Read
Advances in sCMOS Camera Technology Benefit Bio Research sCMOS, cell analysis, imaging24 Jul 2014Read
Rolling Shutter vs. Global Shutter: CCD and CMOS Sensor Architecture and Readout Modes microscopy24 Jul 2014Read
An Automated, High-Throughput Library Construction Protocol with Benefits for Low-Input Applications library preparation23 Jul 2014Read
Improving Next-Generation Sequencing Library Quantification Using KAPA Library Quantification Kits DNA, library preparation23 Jul 2014Read
Optimizing Analytical Performance and Extending the Application Range of Thermal Desorption for Monitoring Air Indoors and Inside Vehicle Cabins air sampling, VOC monitoring, thermal desorption23 Jul 2014Read
Use of Diffusive Samplers with TD–GC Analysis for Monitoring VOCs in Ambient Air air sampling, VOC monitoring, gas analysis23 Jul 2014Read
Principles of Diffusive Monitoring air sampling, absorbance reader, VOC monitoring23 Jul 2014Read
Using Thermal Desorption for the Quality Control of Products and Raw Materials air sampling, VOC monitoring, gas analysis23 Jul 2014Read

Popular tags in SelectScience


Buying guides