Application of the Mirrorball High-Sensitivity Cytometer to Multiplexed Assays for Antibody Drug Discovery
28 August 2015
Highly sensitive, high-throughput assay technologies are required for the identification of antibody therapeutics. In this case study, England, E. et al from MedImmune evaluated a selection of typical cell- and bead-based assays that demonstrate how the multilaser capability of the mirrorball can be exploited to develop highly sensitive multiplex assays. This allows for identifying antibody cross-reactivity and selectivity but significantly without the loss of throughput. In this study the multiplexing of two antibody–cell binding assays (using different dyes) and three cytokine quantitation bead assays (using the same dye at different intensities) was developed.