Increase Column Performance and Lifetime in Peptide and Protein Purification Using Aggressive Wash Conditions on the Ultra-Stable Gemini® Sorbents
30 Oct 2014

Gemini sorbents were specifically developed for chromatographic conditions that demand a wide pH range (pH 1-12). This application demonstrates how the high pH stability of the Gemini product line can be used to extend column lifetime by column regeneration using aggressive alkaline washes (1 M sodium hydroxide in alcohol 1:1 (V/V)). Column regeneration using caustic washes is especially useful in the case of peptide and protein applications where aggregation and impurities tend to accumulate and generate high backpressures. Combining the high pH stability of Gemini particles with the award winning Axia™ pre-packed preparative column will provide longer lifetime and higher performance for the preparative purification of peptide and proteins.

Show    per page
1 of 4     Page  1 2 3  ... Next 
Determination of Uronic Acids and Wood Sugars in Wood-Based Hydrolysates biofuels, wood analysis24 Oct 2014Read
Traditional Chinese Medicine: HPLC Applications Notebook chromatography, HPLC 201420 Oct 2014Read
Automated Purification and Analytical Reinjection of a Small Molecule Drug, Probenecid, on a Gilson LC/MS Dual Function System HPLC method development, method development, Mass Spectrometry24 Sep 2014Read
Quantitative Determination of 26 Aromatic Amines Derived from Banned AZO Dyes in Textiles through the use of LC, Tandem MS, and Identification of Some Structural Isomers amines, Mass Spectrometry, dyes16 Sep 2014Read
Determination of Sudan Dyes and Pesticide Residues in Chili Oleoresin: Optimized Sample Preparation Prior to LC-MS/MS and GC-MS/MS Analysis pesticides12 Sep 2014Read
Determination of Pesticide Residues in Chili Oleoresin: Optimized Sample Preparation Prior to LC-MS/MS and GC-MS/MS Analysis pesticides12 Sep 2014Read
A Novel 6 × 5 Peptide Mixture for Instrument Performance Monitoring method development, peptides9 Sep 2014Read
Assessment of Bisphenol A Specific Migration from Packaging Materials into Food Simulants Using UHPLC-MS/MS and LC with Fluorescence Detection food packaging5 Sep 2014Read
Comprehensive 2D-LC Analysis of Chinese Herbal Medicine active pharmaceuticals, 2D chromatography, pharmaceuticals5 Sep 2014Read
LC-MS/MS Screening of 64 New Psychoactive Substances Using Dried Blood Spots drugs of abuse5 Sep 2014Read

Popular tags in SelectScience