Identification of 1α,25(OH)2-Vitamin D2 and D3 in Serum Samples Using the AB SCIEX Triple Quad™ 5500 System
19 Dec 2014

This application note describes a novel LC/MS/MS method for the determination of dihydroxyvitamin D3 and D2 (DHVD3 and DHVD2) from extracted human serum samples. The key features of the method are a 2D chromatographic separation followed by MRM detection of the DHVD species as their lithium adducts. The use of the lithium DHVD adduct allows for low levels of detection without the use of derivatizing agents thereby greatly simplifying the analytical workflow.

Show    per page
7 of 25     Page  ... 7 8 9  ... Next 
The MED64 Multi‐Electrode Array System human cardiomyocytes, stem cell research, electrophysiology19 Nov 2014Read
Automation of Genomic DNA Isolation with Nucleic Acid Isolation Workstation from Aurora Biomed DNA extraction, liquid handling workstation19 Nov 2014Read
Detection of PARP-Induced ADP-Ribosylation Using the CLARIOstar® Microplate Reader automation, chemiluminescence, assay development18 Nov 2014Read
Evaluation of Cryopreserved Bacmam Transduced Aequorin Cells in 384- and 1536-Well Suspension Cell Assays Using the Aequorin Option for the FLIPRTETRA® System calcium, cryopreservation, high throughput18 Nov 2014Read
The Case for CASE—Saving Time and Effort with Computer-Assisted Structure Elucidation in Routine Analysis protein structure18 Nov 2014Read
Analysis of Skeletal Remains Using Deep mtDNA Sequencing mitochondria, forensic investigation17 Nov 2014Read
Separation of Native Monoclonal Antibodies and Identification of Charge Variants Using the Agilent 3100 OFFGEL Fractionator, Agilent 2100 Bioanalyzer and Agilent LC/MS Systems monoclonal antibodies, quality control, post-translational modifications17 Nov 2014Read
Sample Reformatting Cost for HPLC Analysis automation17 Nov 2014Read
Miniaturizing siRNA Transfection Using the Echo® Liquid Handler liquid handling workstation, siRNA17 Nov 2014Read
Quantitation of 250 Pesticides in Fruit Juices with Positive/Negative Switching LC-MS/MS triple quadrupole MS, food testing14 Nov 2014Read

Popular tags in SelectScience