Identification of 1α,25(OH)2-Vitamin D2 and D3 in Serum Samples Using the AB SCIEX Triple Quad™ 5500 System
19 Dec 2014
This application note describes a novel LC/MS/MS method for the determination of dihydroxyvitamin D3 and D2 (DHVD3 and DHVD2) from extracted human serum samples. The key features of the method are a 2D chromatographic separation followed by MRM detection of the DHVD species as their lithium adducts. The use of the lithium DHVD adduct allows for low levels of detection without the use of derivatizing agents thereby greatly simplifying the analytical workflow.