Identification of 1α,25(OH)2-Vitamin D2 and D3 in Serum Samples Using the AB SCIEX Triple Quad™ 5500 System
19 Dec 2014

This application note describes a novel LC/MS/MS method for the determination of dihydroxyvitamin D3 and D2 (DHVD3 and DHVD2) from extracted human serum samples. The key features of the method are a 2D chromatographic separation followed by MRM detection of the DHVD species as their lithium adducts. The use of the lithium DHVD adduct allows for low levels of detection without the use of derivatizing agents thereby greatly simplifying the analytical workflow.

Show    per page
1 of 25     Page  1 2 3  ... Next 
Amyloid-β Binding Alcohol Dehydrogenase (ABAD), Estradiol and Mitochondrial Dysfunction in Alzheimer's Disease alzheimers disease, inhibitors19 Dec 2014Read
Detection of Human IgG Using a Luminescence-based Proximity Assay chemiluminescence, assay development, biomarker discovery19 Dec 2014Read
Below amol ATP Detection Limit with Sirius L and ATP Reagent SS from BioThema ATP monitoring18 Dec 2014Read
Masking Method Reveals New Aspects of Neuron Morphology 3D imaging, neurons17 Dec 2014Read
High-Throughput Screening of Deubiquitylase Enzyme (DUB) Activity/Specificity and Inhibitor Screening by MALDI-TOF Mass Spectrometry MALDI Imaging17 Dec 2014Read
Applied Robotics for Enhanced Throughput Options in Microscopy as Demonstrated by Automated Tissue Microarray (TMA) Imaging tissue processing, high throughput16 Dec 2014Read
Mouse Diabetes Multiplex Metabolic Biomarkers Assay diabetes, animal models16 Dec 2014Read
Reliable Purification of PCR Products with High Yield and Purity Using the MACHEREY-NAGEL NucleoSpin® 96 PCR Clean-up kit on a Freedom EVO® Platform high throughput, PCR automation16 Dec 2014Read
Automated MAb Workflow: from Harvest Cell Culture to Intact Mass Analysis of Variants monoclonal antibodies, HPLC method development16 Dec 2014Read
Corning® Epic® Label-Free Cell Based Assay: Development of a One-Day Assay for Adherent Cells Label-Free Analysis16 Dec 2014Read

Popular tags in SelectScience