Thermal Stability Buffer Screening of Therapeutic Antibodies
22 February 2017

Therapeutic antibodies require optimal formulations to ensure long-term stability and in the drug development process, the thermal stability of the antibody in a range of buffer formulations is routinely tested. This application note presents a label-free DSF (Differential Scanning Fluorimetry) technique using the NanoTemper Prometheus NT.48 as method to screen therapeutic antibody buffers. The Prometheus NT.48 can quickly obtain high quality thermal unfolding data for antibodies over a large range of antibody concentrations.