Separation of Steviol Glycosides
27 Jan 2015

A robust, selective, and sensitive separation method for the determination of four steviol glycosides in stevia plant matrix is demonstrated in this application note. In addition to the two calibrated substances, two more glycosides could be found in stevia plant extracts by LC-MS. These two substances were quantified by means of a stevioside calibration. Small HPLC columns and the use of a KNAUER Smartline HPLC system provide a fast and easy way for the separation of complex stevia extracts.

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