Transfer of USP Cholecalciferol Normal-phase HPLC Method to SFC Using the Agilent 1260 Infinity Hybrid SFC/UHPLC System
15 Mar 2013

This Application Note describes the conversion of the United States Pharmacopeia (USP) normal-phase HPLC method for cholecalciferol assay to an SFC method using the Agilent 1260 Infinity Hybrid SFC/UHPLC System. The results show that the SFC method meets the system suitability criteria, is 2x faster, and achieves 26x lower solvent costs compared to the USP method.

Agilent Technologies


Tags: USP method, USP

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