Application Note: Flow Cytometric Analysis of Multiple Signaling Pathways in LPS Stimulated Human Peripheral Blood Monocytes
6 Mar 2015

The availability of antibodies specifically reactive with phospho-epitopes characteristic of activated signaling proteins provides specific probes that can be used to simultaneously monitor multiple signal transduction pathways. This poster describes an optimized protocol for specific fixation and permeabilization techniques to monitor signaling pathways in blood and bone marrow samples.