Advancing Host Cell Protein Analyses Through the Combined Use of Microscale 2D RP/RP with CSH C18 and Ion Mobility Enabled MS Detection
26 Feb 2015

In this application note, recent advances in microscale peptide chromatography are applied to HCP analysis. The novel charge-surface-modified C18 material, known as CSH C18, which can produce high peptide peak capacities even at high mass loads, is used in the format of a 15K psi capable 300 μm I.D. column along with an ACQUITY UPLC M-Class based 2D-RP/RP system and SYNAPT HDMSE detection to provide a robust analytical workflow for the identification of low concentrations of HCP impurities at single digit PPM levels (parts per million, or ng/mg).

Show    per page
1 of 21     Page  1 2 3  ... Next 
Extraction of 25-OH-Vitamin D2 and D3 from Serum by Novum™ Simplified Liquid Extraction and LC/MS/MS Analysis Using a Kinetex® HPLC/UHPLC Column Sample Preparation23 Feb 2015Read
A Simple and Effective Method for Simultaneous HPLC Quantification of Vitamin B1 and Vitamin B6 from Whole Blood B Vitamins, Blood Analysis23 Feb 2015Read
LC/MS/MS Analysis of Oxytocin and ARG-Vasopressin in Human Plasma/Serum Using Strata™-X-CW Solid Phase Extraction and a Luna® PFP(2) HPLC Column Hormones23 Feb 2015Read
Effectiveness of the AC Extraction Plate for Protein Removal Protein Purification20 Feb 2015Read
Structural Comparison of Infliximab and a Biosimilar via Subunit Analysis Using the Waters Biopharmaceutical Platform with UNIFI Monoclonal Antibodies, Biosimilars19 Feb 2015Read
Analysis of a Peptide Mixture using the LCMS-8050 Triple Quadrupole Mass Spectrometer Peptides16 Feb 2015Read
UPLC-MS/MS Analysis of Aldosterone in Plasma for Clinical Research Hormones, Plasma16 Feb 2015Read
Metabolomics of Broccoli Sprouts Using UPLC with Ion Mobility Enabled LC/MSE and TransOmics Informatics Metabolomics13 Feb 2015Read
Analysis of Serum Adrenal Steroids Using the ACQUITY UPLC I-Class with Xevo TQD for Clinical Research Steroids9 Feb 2015Read
Performance of ACQUITY UPLC M-Class in Proteomics Nanoscale Applications Proteomics6 Feb 2015Read

Popular tags in SelectScience