Determination of Phospholipids in Lecithin Using the KNAUER Smartline HPLC with ELSD Detector
23 Oct 2014
An analysis of the phospholipids in raw lecithin was demonstrated using normal-phase HPLC with an Evaporative Light Scattering Detector (ELSD). Lecithin is a natural substance found in fatty animal tissue such as egg yolk, as well as in plant tissues. It is composed of phosphoric acid, choline, fatty acids, glycerol, glycolipids, triglycerides, and phospholipids. Lecithin is commonly used as an additive in foods, cosmetics and even pharmaceuticals. Due to its complex composition and natural source, chemical analysis is essential. In this application the phospholipids in lecithin have been separated into their major components phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) using normal-phase HPLC. Since phospholipids absorb UV light only very weakly, evaporative light scattering detection (ELSD) was used.