Regeneration and Cleaning of Eshmuno® HCX Chromatography Resin: A Screening Study
22 October 2014

Eshmuno® HCX is a high salt-tolerant, multimodal chromatography resin designed for fast and efficient direct capture of proteins. Eshmuno® HCX resin offers outstanding performance results in typical ion exchange and flow-through applications at higher salt levels and binds solutes in multiple modes, including electrostatic, hydrophobic and/or hydrogen bonding. While multi-modal adsorption facilitates capture at high conductivities, it also makes it necessary to optimize resin cleaning and regeneration to account for the multiple modalities of adsorption. This application note identifies the most promising cleaning and regenerating solutions for Eshmuno® HCX resin.

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