Improving the Chromatographic Separation of DMB-Labeled Sialic Acids for the Comparison of Biosimilars to Reference Materials
18 January 2016

Sialic acids are important because they affect bioavailability, function, stability, and metabolism of glycoproteins. A new HPLC method employing an Ascentis® Express RP-Amide column was developed to determine DMB-labeled NGNA and NANA sialic acids released from biotherapeutic proteins. This article explains the many advantages this method offers over the original C18 method.