Comparison of Various Methods of Retrovirus-Mediated Gene Transduction in Human Stem Cells
30 Sep 2014

RetroNectin® (Recombinant Human Fibronectin Fragment) can dramatically increase retroviral and lentiviral transduction efficiency in hard-to-infect cell types and hematopoietic stem cells. This application note presents a comparison of three different methods (RetroNectin Reagent, Polybrene, and Protamine) for measuring the expression of a retrovirally-delivered recombinant fluorescent protein in stem cells.

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