1-day Chromatrap® Spin Column Assay for Rapid ChIP Analyses
24 Oct 2014

Chromatin immunoprecipitation (ChIP) is a versatile tool for identifying protein-DNA associations. The procedure can, however, be time consuming, requiring tedious optimization of several reaction conditions and up to two overnight incubations (first for antibody binding to the target and then for reversing the cross-linked immunoprecipitated DNA). The Chromatrap® 1-day ChIP method presents a quicker alternative that allows the IP assay and data analysis to be completed in just 6 hours enabling more ChIP assays to be performed daily. This application note demonstrates the use of the rapid Chromatrap® 1-day spin column protocol for analyzing the high abundant antibody binding efficiency of RNA Pol II onto target genes of interest (GAPDH and β-globin) in endometrial epithelial, cervical and leukemic cancer cells.

Show    per page
4 of 31     Page  ... 4 5 6  ... Next 
Precision Pipetting of Problem Liquids Using the MICROMAN® E Pipette contamination prevention, sample handling17 Oct 2014Read
Magnetic Bead Based Soil DNA Isolation Using the epMotion® and ClearMag™ Technology magnetic beads, DNA extraction, high throughput16 Oct 2014Read
Specific Curing of Encapsulant Material with BINDER Drying and Heating Chambers temperature control, LED16 Oct 2014Read
Top 10 Things to Consider When migrating to the Mastersizer 3000 from the Mastersizer 2000 particle size analysis16 Oct 2014Read
Vi-CELL® XR Cell Viability Analyzer Integration with Biomek® NXP cell viability, liquid handling workstation16 Oct 2014Read
Development of Low Extractable Syringe Filters for Liquid Chromatography/Mass Spectrometry Applications MS syringe filters, syringe filtration15 Oct 2014Read
Cable Testing with BINDER Ovens temperature control15 Oct 2014Read
Test Systems for Sounding Out Building Materials15 Oct 2014Read
Protein Coatings Facilitate the Differentiation of Reconstructed Epithelia in ThinCert™ Cell Culture Inserts cellular growth14 Oct 2014Read
Semi-Automated Genomic DNA Extraction from Plant with CyBi®-SELMA 96/1000 μl Using NucleoSpin® 96 Plant II Kit DNA extraction, plant science14 Oct 2014Read

Popular tags in SelectScience


Buying guides