Transcription Factor Binding Studies with IP-Star® Compact Automated System from Diagenode
29 Oct 2014

ChIP experiments require long and multi-step protocols, and the risk of introducing inconsistency is very high. The goal of this experiment was to confirm the findings from ChIP sequencing by using an automated ChIP technology in order to reduce all inconsistency introduced by the pipetting variations and human variability. This application note presents high sensitivity automated ChIP experiments to study transcriptional regulation and siRNA technology. The study used the Bioruptor UCD-300-TO with associated reagents for chromatin preparation and the SX-8G IP-Star® Compact Automated System with associated reagents for ChIP and an ETO antibody for the immunoprecipitation step.

Show    per page
1 of 6     Page  1 2 3  ... Next 
Chromatin Preparation From Tissues for Chromatin Immunoprecipitation Using Diagenode’s Chromatin Shearing Optimization Kit - Low SDS and Bioruptor® DNA shearing, immunoprecipitation, chromatin29 Oct 2014Read
Good Westerns Gone Bad: Tips to Make Your NIR Western Blot Great24 Oct 2014Read
Eurofins’ Adaption and Optimization Software “GENEius” in Comparison to Other Optimization Algorithms protein expression, genetic engineering, E coli21 Oct 2014Read
How Droplet Digital PCR Has Revolutionized Non-Invasive Plasma-Based Detection of Mutations cancer diagnostics15 Oct 2014Read
Microinjection into the Gonads of C. elegans Using Eppendorf InjectMan® 4 regulatory, genetic engineering, gene regulation14 Oct 2014Read
Lysate Clearance Using the AcroPrep™ Advance Filter Plate molecular biology13 Oct 2014Read
Five Ways That Multiplex Technology Accelerates Cancer Research cancer diagnostics10 Oct 2014Read
Use of DNA Ligation Kit, Mighty Mix for Cohesive- and Blunt- End Ligation transfection, E coli7 Oct 2014Read
T Cell Expansion in the Presence of RetroNectin® Reagent immunology30 Sep 2014Read
Comparison of Various Methods of Retrovirus-Mediated Gene Transduction in Human Stem Cells stem cell research30 Sep 2014Read

Popular tags in SelectScience

Buying guides