A GloMax® Multi Microplate Luminometer Method for Dual-Luciferase® Reporter Assay System
24 June 2015
The Dual-Luciferase® Reporter Assay (DLR) from Promega is ideal as a dual genetic reporting luminescence-based assay system for quantitation of gene expression. Two different luciferase reporter enzymes, the firefly (Photinus pyralis) and Renilla (sea pansy or Renilla reniformis), are expressed simultaneously in each cell creating an efficient means of performing two reporter assays in a single sample. The GloMax® Multi Microplate Luminometer with dual injectors provides a convenient, rapid, and sensitive method for quantifying gene expression when used with the Dual-Luciferase® Reporter (DLR) Assay kit. The superior sensitivity and wide dynamic range of the GloMax® Multi Microplate Luminometer make it highly suited for the Dual-Luciferase® Reporter (DLR) Assay application. The GloMax® Multi Microplate Luminometer detects less than 1x10-19 moles of firefly luciferase enzyme using Luciferase Assay Reagent II (LAR II) and 1x10-18 moles of Renilla enzyme using Stop & Glo® Reagent. The GloMax® Multi Microplate Luminometer offers superior sensitivity and dynamic range for luminescence detection, such as the luminescence based dual-reporter assays.