Characterizing Epigenetic Protein Interactions using Isothermal Titration Calorimetry
18 July 2016
This white paper provides an introduction to epigenetics and describes how Isothermal Titration Calorimetry (ITC) can be used to characterize binding interactions involving proteins, nucleic acids and lipids. ITC measures the heat changes associated with binding events, determining the binding affinity of any biomolecular interaction. ITC is established in life sciences as the “gold standard” method for the study of binding processes, since it is label-free, sensitive, versatile, and can be used with different materials in a variety of buffers.