Accurate Cytotoxicity and Proliferation Determination: Advantages of a High-Throughput Phenotypic Approach Over ATP-Luminescence Assays
15 July 2016

This application note shows the development and implementation of a cost effective, no-wash HCA assay to simultaneously report the cell number, percentage of live cells and cell cycle phase distribution as markers of proliferation and viability. This assay can be applied to high density plate formats, and be imaged and analyzed in only 8 minutes per plate using the acumen Cellista laser scanning imaging cytometer from TTP Labtech.