Ion-Exchange Chromatography for Biomolecule Analysis
28 January 2015
Ion-exchange is a widely used method for separating biomolecules based on differences in ionic charge. It is a mild, non-denaturing technique that does not require organic solvents and is therefore frequently used for characterization of proteins in their native or active form, and for purification. This guide discusses ion-exchange (IEX) chromatography, column selection choices, important mobile phase considerations, general rules of thumb for using IEX, instrument considerations, and more.