Supercritical Fluid Chromatography Mass Spectrometry
27 Nov 2014

This application note describes the development of a method for the Agilent 1260 Infinity Analytical SFC Solution for the separation of a multi-pesticide sample in a short run time, which is faster than typical HPLC methods. Connecting the SFC to the Agilent 6460 Triple Quadrupole LC/MS System and the optimization of the necessary makeup flow is discussed, as well as MRM and MS source parameters. For the final method, important performance parameters such as limits of detection (LOD), limits of quantification (LOQ), retention time, and area RSD are determined.

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