10-Color Analysis of T Cell Subpopulations
25 November 2015
Flow cytometry is an essential tool for analysis of multiple qualitative and quantitative characteristics of a single cell in a mixed cell population. It is widely used in life science research, drug discovery and plant and agricultural science research to assess cell cycle, cell proliferation, and DNA content. It is also increasingly applied in routine clinical laboratories for the diagnosis, prognosis and monitoring of diseases. Understanding the function and interaction of the molecular and cellular components of the immune system demands multiparameter analysis. This application note demonstrates the use of the NovoCyte Flow Cytometer to study functional and phenotypic properties of these subpopulations based on biological function and cell surface antigen expression.