Multiplexed FLISA Assays on mirrorball®
24 August 2015

Enzyme-linked immunosorbant assays (ELISA) can be used in multiple stages of the drug discovery process to help identify and measure the cellular responses to therapeutic or toxic molecules. Although ELISAs are very sensitive, they are time-consuming, require multiple separate wash steps and incubation steps and are not amenable to multiplexing. This application note demonstrates the transfer of standard commercially available colorimetric IL-8 and IL-6 ELISA kits onto a no-wash bead-based assay format on the mirrorball, FLISA (fluorescence-linked immunosorbant assay). The resulting FLISA format offers significant process efficiency improvements through use of a single incubation step, without the requirement for wash steps, and is amenable to multiplexing.