1-day Chromatrap® Spin Column Assay for Rapid ChIP Analyses
24 Oct 2014

Chromatin immunoprecipitation (ChIP) is a versatile tool for identifying protein-DNA associations. The procedure can, however, be time consuming, requiring tedious optimization of several reaction conditions and up to two overnight incubations (first for antibody binding to the target and then for reversing the cross-linked immunoprecipitated DNA). The Chromatrap® 1-day ChIP method presents a quicker alternative that allows the IP assay and data analysis to be completed in just 6 hours enabling more ChIP assays to be performed daily. This application note demonstrates the use of the rapid Chromatrap® 1-day spin column protocol for analyzing the high abundant antibody binding efficiency of RNA Pol II onto target genes of interest (GAPDH and β-globin) in endometrial epithelial, cervical and leukemic cancer cells.

Show    per page
1 of 8     Page  1 2 3  ... Next 
Bacterial Cell Disruption Using Bioruptor® Plus DNA shearing, lysis20 Oct 2014Read
Precise Sizing and SMRT Sequencing Offer Unprecedented Read Length for Clinical Studies size selection, epigenetics, high throughput20 Oct 2014Read
KASP™ Genotyping Chemistry: How it Works SNP genotyping16 Oct 2014Read
KASP™ Genotyping Technology Accelerates Poultry Breeding Program at Hy-Line International SNP genotyping16 Oct 2014Read
How Droplet Digital PCR Has Revolutionized Non-Invasive Plasma-Based Detection of Mutations cancer diagnostics15 Oct 2014Read
Automation of ChIP-Seq Library Preparation for Next Generation Sequencing on the epMotion® 5075 TMX ChIP-sequencing, library preparation, NGS14 Oct 2014Read
Quality Control of High-Throughput Library Construction Pipeline for KAPA HTP Library Using an Agilent 2200 TapeStation library generation, library preparation8 Oct 2014Read
Amplification of β-globin, K-ras 12, p53 (exon 5, exon 6) Genes from 10-Year-Old Paraffin Sections cancer, p536 Oct 2014Read
Double Stranded DNA Quantitation Using Promega QuantiFluor® Dyes automation, DNA2 Oct 2014Read
Automated Illumina TruSeq® Nano DNA Sample Preparation automation, library preparation, NGS2 Oct 2014Read

Popular tags in SelectScience


Buying guides