High-Throughput 96-well and 384-well nucleic DNA purification with MagSi-DNA cleanFIX
23 Oct 2014

In many genomics applications, purification of DNA fragments is required to prevent downstream interference with analysis methods. MagSi-DNA cleanFIX offers a fast and efficient solution for purifying DNA fragments, removing all unwanted side products and reagents, such as single nucleotides, terminator dyes, primers and primer dimers, enzymes, buffers and salts. MagSi-DNA cleanFIX enables a combination of PCR clean-up and dye terminator removal from sequence reaction mixes with a single product. The kit uses magnetic particle technology, and is easily automated because no columns or centrifugation steps are involved. This application note describes how MagSi-DNA cleanFIX is used on JANUS® Automated Workstations for high throughput nucleic acid purification. The goal was to set-up a 384-well clean-up automated solution, increasing throughput, reliability and data robustness while reducing reagent consumption and disposable costs.

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