Chromatrap® Enzymatic Shearing Kit Isolates High Quality Chromatin for Excellent Target Enrichment
30 Oct 2014

Isolation of good quality, suitably fragmented chromatin is the most important prerequisite for successful chromatin immunoprecipitation. Chromatin can be sheared enzymatically or by mechanical methods such as sonication. Enzymatic shearing can be advantageous where expensive sonication equipment is not available or where native chromatin is to be examined. Illustrated here in this short application note is the effectiveness of the Chromatrap® Enzymatic Shearing Kit in the fragmentation and isolation of high quality ChIP grade chromatin. We also highlight comparable data for enzymatic versus sonication using Chromatrap® ChIP kits.

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