Detection of Treg Subset from Mouse Splenocytes
3 September 2015
Regulatory T cells (Tregs) are a small subset of CD4+CD25highFoxp3+ T cells. Tregs negatively regulate immune responses and play a pivotal role in the homeostasis of the immune system. These regulatory T cells have emerged as key players in the development and maintenance of peripheral immune tolerance, and are potential therapeutic targets. Fluctuation in Treg populations have been well documented in many diseases, and flow cytometry is an important tool in studying Treg. This application note demonstrates the use of the NovoCyte flow cytometer in the identification of Treg subset from mouse splenocytes.