Determining Metabolic Switch in Induced Pluripotent Stem Cells (iPSCs)
29 Aug 2014

In this application note a Seahorse XF Extracellular Flux Analyzer was used to assess the metabolic changes of iPSCs derived from human fibroblasts using the XF Cell Mito Stress Test and XF Glycolysis Stress Test kits. It was determined that the metabolic switch occurs early in the nuclear reprogramming process and both HIF1α and HIF2α have sequential roles in the metabolic switch during iPSC induction.

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