Detecting Human Circulating Endothelial Cells Using the Attune® Acoustic Focusing Cytometer
17 Sep 2014

This application note describes a method for detecting human circulating endothelial cells using the Attune® Acoustic Focusing Cytometer. The method includes a number of conventional ways to improve rare-event detection: a blocking step, a viability stain (SYTOX® AADvanced™ Dead Cell Stain), and the use of a dump channel to eliminate unwanted cells and decrease background fluorescence.

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