Analysis of Intact Macromolecular Assemblies On A Bench Top Orbitrap MS System
1 December 2015
This application note demonstrates the measurement of proteins in native like state to study the biologically active conformations. Intact proteins are routinely measured using ESI-MS instrumentation under acidic, denaturing conditions, destroying large, non-covalent protein assemblies and substrate bound complexes. Under native conditions, fully active protein assemblies can be studied, but these experiments are challenging due to the limited surface area of protein complexes for protonation at physiological pH.