Detection of Binding-Induced Conformational Changes by Surface Acoustic Wave

5 April 2016

This application note investigates binding-induced conformational changes in E. coli, measured using Surface Acoustic Wave (SAW). Binding of maltose to immobilized MBP results in a quantifiable signal response, not only for a change in mass, but also for the marked conformational transition between the two structural MBP domains. Using appropriate controls this study not only confirms the accessibility of binding induced conformational changes with SAW, but also amplitude-based Kd extraction. The NanoTemper Technologies Seismos NT.X SAW biosensor allows assessment of protein-small molecule interactions with high sensitivity.