Real-Time Monitoring of Genetically Encoded Redox Probes in Mammalian Cell Monolayers
21 August 2015
This application note demonstrates that the PHERAstar FS reader enables roGFP2-based measurements in mammalian cell monolayers grown in 96-well imaging plates. Redox processes play an important role in cellular physiology and pathology. A particularly powerful tool for the monitoring of cellular redox changes are genetically-encoded biosensors based on redox sensitive green fluorescent protein (roGFP). RoGFPs contain two cysteine residues engineered to be present on the surface of the protein b-barrel, which are capable of forming a disulphide bond. RoGFP can be made to respond to specific redox species via the genetic fusion of appropriate redox enzymes.