Detection of Human IgG Using a Luminescence-based Proximity Assay
19 Dec 2014

This application note describes the use of the Synergy Neo Multi-Mode Microplate Reader in conjunction with the Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL) assay technology to quantitate human IgG. (SPARCL) technology is a proximity dependent, chemiluminescent detection technique. In the assay, a chemiluminescent substrate (acridan) is brought into the proximity of an oxidative enzyme (horseradish peroxidase, HRP) through the specific antigen/antibody interaction. Once in close proximity adding the “trigger” reagent results in a flash luminescent signal that is proportional to the amount of analyte.

Show    per page
1 of 7     Page  1 2 3  ... Next 
Mouse Diabetes Multiplex Metabolic Biomarkers Assay diabetes, animal models16 Dec 2014Read
An Introduction to Immunotherapy and the Promise of Tissue Phenomics® immunology, cancer, phenome3 Dec 2014Read
InnoScan® 710IR Infrared Detection for Optimal Scan of Nitrocellulose-Based Protein Arrays protein analysis26 Nov 2014Read
Fighting Autoimmune Disease the Need for a Personalized Approach companion diagnostics, autoimmunity19 Nov 2014Read
InnoScan 710-IR Scanner Excels at Cytokine Detection in Conjunction with pGOLD Chips cytokines, immunology19 Nov 2014Read
Using Genetix ClonePix FL for Hybridoma screening18 Nov 2014Read
NIR RPPA Detection Together with Dynamic Range Extension (XDR) Function in the Innoscan 710-IR Scanner Makes RPPA Detection Cheaper By 90 Percent biomarker discovery, RPPA12 Nov 2014Read
Protein-Ligand Binding Measurements Using Fluorescence Polarization epigenetics, post-translational modifications10 Nov 2014Read
The Role of Multiplex Assays in Analyzing the Cytokine Storm immunoassay, cytokines, multiplex7 Nov 2014Read
Viruses and Viral Vaccines: Characterization by Nanoparticle Tracking Analysis viruses, nanoparticles31 Oct 2014Read

Popular tags in SelectScience


Buying Guides