Optimization of Enzyme Reactions for Screening and Profiling Wild Type EZH2 with the Transcreener EPIGEN Methyltransferase Assay
31 Oct 2014

Post translational modification of histone proteins by Histone Methyltransferases plays an important role in gene regulation. Misregulation of these enzymes has been implicated in initiation and progression of several diseases, including cancer. EZH2 is the catalytic component of the polycomb group repressive Complex (PRC2), that selectively methylates histone H3 lysine 27 (H3K27). Numerous studies have shown that EZH2 is overexpressed in prostate, breast, bladder, brain, and other tumor types and is recognized as a molecular marker for cancer progression and aggressiveness. This poster describes the development, optimization and validation of Transcreener EPIGEN Methyltransferase assay for EZH2. To aid in drug discovery Bellbrook Labs developed an EZH2 Transzyme Methyltransferase Assay kit which contains enzyme, substrate and the Epigen Methyltransferase kit.

Show    per page
3 of 4     Page  1 2 3  ... Next 
Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses in vitro, 3d cell culture, fluorescence based assay4 Dec 2013Read
A Fully Automated System for Storage of Compounds and Assay Plate Production automation, assay development, compound storage4 Dec 2013Read
Cardiotoxicity of iCell® Cardiomyocytes Using the CyBi®-SELMA 96, 250 μl Semi-automated Pipettor cell analysis, cardiotoxicity, liquid handling workstation3 Dec 2013Read
A High-Throughput, Homogeneous, FRET-Based Assay to Detect Bacterial Membrane-Bound Enzyme assay development, screening, FRET3 Dec 2013Read
HP D300 Digital Dispenser Creates a Dose Response Curve for Cell-Based and Biochemical Assay drug discovery, Tecan20 Nov 2013Read
A Practical Guide to High Resolution Melt Analysis Genotyping high resolution melt, qPCR19 Nov 2013Read
Imaging of BacMam Transfected U-2 OS Cells: Optimization of Transfection Conditions Using the Cytation™3 Cell Imaging Multi-Mode Reader and Gen5™ Data Analysis Software digital microscopy, cell imaging, cell-based platform9 Oct 2013Read
Automated, High Throughput, HTRF®-Based Detection of Histone Methyltransferase and Demethylase Enzyme Activity fluorescence based assay, histone modification, epigenetics3 Oct 2013Read
Using CombiCult® in combination with ClonePixTM Technology to Select Media Components which Induce MSC Cells to Secrete High Levels of VEGF stem cell research, assay development, screening1 Oct 2013Read
Nanoparticle Tracking Analysis: A Review of Applications and Usage 2010 - 2012 Dynamic light scattering, nanoparticles3 Sep 2013Read

Popular tags in SelectScience


Buying guides