TR-FRET Based Transcreener AMP2/GMP2 Assay for Screening Ligases, Synthetases and Phosphodiesterases
22 Oct 2014

The exploration of more extensive drug target space by pharmaceutical and academic labs is creating a need for HTS compatible methods to detect diverse enzymes. The Transcreener AMP2/GMP2 Assay is simple, single step, homogenous, amenable to HTS and enables the detection of any AMP/GMP producing enzyme while using any precursor substrate. Importantly, the assay relies on direct immunodetection of AMP and GMP without using coupling enzymes, which eliminates the need for counter-screening. The readout options have been extended to include far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) in addition to the original fluorescence polarization based assay. This poster describes the development, optimization and validation of the Transcreener AMP2/GMP2 TR-FRET Assay, including detection of different classes of AMP/GMP-producing enzymes.

Show    per page
1 of 3     Page  1 2 3 Next 
Diverse Applications of the Novel Small, Bright NanoLuc Luciferase GPCR, reporter assay, luciferase10 Sep 2014Read
A Bioluminescent Cell Viability Assay Optimized for 3D Microtissues 3d cell culture, ATP monitoring, microtissue9 Sep 2014Read
IMAP Phosphodiesterase Assays on SpectraMax Multi-Mode Microplate Readers assay development, kinase assays4 Sep 2014Read
Liquid Perfusion without the Overhead: Fast, Serial Liquid Replacement For PAM Assay Development assay development3 Sep 2014Read
Potential Cholestatic Drugs Assessed by Membrane Transport Vesicle Assays as Well as Bile Canaliculi Inhibition in Primary Hepatocytes assay development, vesicles, Hepatocytes2 Sep 2014Read
Development of an in vitro Multiwell Cardiovascular Multielectrode Array (MEA) Toxicity Assay with Human IPS Cell Derived Cardiomyocytes drug development, cardiotoxicity, Cardiomyocytes2 Sep 2014Read
Real Time Calcium Flux Measurements in iPSC 3d cell culture, fluorescence based assay, animal models27 Aug 2014Read
Rapid Production and Size Assessment of Embryoid Bodies (EB) 3d cell culture, cytotoxicity, high throughput22 Jul 2014Read
Multiplexed Viability, Cytotoxicity and Apoptosis Assays for Cell-Based Screening cytotoxicity, liquid handling workstation, assay development21 Jul 2014Read
ADME and Cytotoxicity Assays – Use of the CyBi® - Well for Reagent Addition and Washing Procedures cytochrome p450 assays, electronic pipette, ADME17 Jul 2014Read

Popular tags in SelectScience

Buying guides