Size-Exclusion Ultra Performance Liquid Chromatography for the Analysis of Covalent High Molecular Weight Insulin
8 Apr 2013Presented in this application note are the advantages that may be realized using a 125Å pore size, sub-2-µm ethylene-bridged hybrid (BEH) silica packing material and Waters UltraPerformance Liquid Chromatography (UPLC) instrumentation for this traditional analysis. Among these advantages are faster run times, higher sensitivity, and higher resolving separations of insulin and covalent insulin HMW, while at the same time greatly reducing acetonitrile containing waste-stream volumes.
Related Products
Request Quote for All Products
ACQUITY UPLC H-Class PLUS Bio System
WatersThe ACQUITY UPLC H-Class PLUS Bio System delivers the benefits of UPLC's resolution, sensitivity, and throughput in a system purpose-built for the analysis of proteins, peptides, nucleic acids, and glycans.
BEH (Ethylene Bridged Hybrid) Particles for ACQUITY UPLC
WatersThe 1.7 µm Ethylene Bridged Hybrid [BEH] particle is one of the key enablers behind UPLC® technology.It is available in three different pore sizes [130Å, 200Å and 300Å] and several bonded phases for reversed-phase and hydrophilic interaction chromatography and is applicable from small molecule to large biopharmaceutical analysis. Due to the intrinsic chemical stability of hybrid particle technology, a wider usable pH range [pH 1-12] can be employed, enabling a versatile and robust separation technology for method development. BEH particle technology is available in HPLC particle sizes [2.5, 3.5, 5 and 10 µm] in the XBridge® family of HPLC columns, enabling seamless transfer between HPLC and UPLC technology platforms.