Peptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases

18 Mar 2013

This application note describes the comparison of a CSH130 C18, BEH130 C18, and a superficially porous C18 column for the LC/MS of an enolase tryptic digest. In addition, the applicability of these columns for separations of polypeptides up to 12 kDa is evaluated.

ACQUITY UPLC H-Class PLUS Bio System

Waters

The ACQUITY UPLC H-Class PLUS Bio System delivers the benefits of UPLC's resolution, sensitivity, and throughput in a system purpose-built for the analysis of proteins, peptides, nucleic acids, and glycans.

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Peptide Mapping and Small Protein Separations with Charged Surface Hybrid (CSH) C18 and TFA-Free Mobile Phases